Figure 6.
Effect of WISP-1 on DNA synthesis. NRK-49F cells were plated in 96-well plates at 3 × 104 in HGDMEM with 10% serum. Twenty-four hours after plating, the medium was changed to HGDMEM with 0.2% serum. WISP-1 was serially diluted in fresh medium and added at the indicated concentrations in a total volume of 70 μl/well. After 18 hr incubation at 37°C, 5 μCi/ml [3H]-thymidine was added for 5 hr. Cells were harvested onto a GF/C filter using Packard's 96-well Filtermate 196 and counted on a top count, microplate scintillation counter (Packard). Values are the means of triplicate wells. The experiment was repeated at least four times with similar results.