Figure 7.

(A) Expression of hWISP-1 mRNA in NRK-49F cells. NRK-49F cells were infected with retroviruses expressing empty pBabe-puro vector or pBabe-hWISP-1 and selected with puromycin for resistant colonies. Stable colonies (>10⁵) were pooled. Northern blot analysis was carried out with 10 μg of total RNA. Full-length mouse WISP-1 cDNA was used as a probe. The blot was rehybridized with a probe for mouse GAPDH gene. (B) WISP-1 expression alters the morphology of NRK–49F fibroblasts. Phase contrast microscopy of polyclonal NRK–vector and NRK–WISP1 fibroblasts cell lines. (1,2) NRK–vector and NRK–WISP1 cells at the same passage number after infection, respectively. (C) WISP-1 expression alters the growth of NRK–49F cells. NRK–vector (●) and NRK–WISP1 (▴) cells (both are of passage 5) were seeded at low density (10⁵/10-cm plate) and monitored for growth. Cell numbers are presented as means ±s.d. of three plates.