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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Oral Oncol. 2011 Jul 6;47(9):831–839. doi: 10.1016/j.oraloncology.2011.06.012

Fig. 6. Binding of rhAMP-18 to CCKBR expressed in 293T cells.

Fig. 6

CCKBR was expressed in 293T cells (293T+CCKBR). Control 293T cells (293T) were transfected with empty pBaBe vectors (top 3 panels). Cells were grown on poly-lysine-coated coverslips. Forty-eight hours later the cells were fixed and incubated with His-tagged or His-and Flag-tagged rhAMP-18 overnight. Expressed CCKBR and bound AMP-18 were detected and co-localized by double immunofluorescence using an anti-CCKBR antibody (blue) and an anti-His antibody (red, middle 3 panels), or anti-Flag antibody (red, bottom 3 panels) followed by staining with appropriate Alexa-conjugated secondary antibodies (purple, merged). The purple merged images suggest colocalization of rhAMP-18 and CCKBR. Representative images are shown from 3 experiments.