Table 1.
TUNEL specifically labels dying cells
Genotype
|
No. of TUNEL-positive cellsa
|
Range of TUNEL-positive cells
|
No. of cell corpsesb
|
---|---|---|---|
Wild type | 1.7 ± 1.3 | 0–4 | 14 ± 1 |
ced-3(n717) | 0.1 ± 0.2 | 0–1 | 0.0 ± 0.0 |
ced-4(n1162) | 0.0 ± 0.0 | 0–1 | 0.0 ± 0.2 |
ced-9(n1950) | 0.1 ± 0.4 | 0–1 | 0.1 ± 0.3 |
TUNEL-positive nuclei in at least 60 embryos of each genotype were counted.
Cell corpses in 15 wild-type embryos and in >50 embryos of each mutant genotype were counted using Nomarski optics. Embryos were scored at the 1.5-fold stage. The data shown are means ± s.e.m.