Table 4.
Mutations in ced-1 and ced-7 reduce TUNEL staining
| Genotype
|
No. of TUNELpositive cells (n = 45)
|
Range of TUNELpositive cells
|
No. of cell corpses (n = 15)
|
|---|---|---|---|
| Wild type | 1.7 ± 1.3 | 0–4 | 14 ± 1 |
| ced-1(e1735) | 0.5 ± 0.7 | 0–3 | 24 ± 3 |
| ced-1(n1995) | 0.3 ± 0.2 | 0–2 | 25 ± 4 |
| ced-7(n2094) | 0.7 ± 0.8 | 0–3 | 34 ± 3 |
| ced-7(n1892) | 0.5 ± 0.4 | 0–2 | 35 ± 5 |
| nuc-1(e1392) | 47.8 ± 4.8 | 38–64 | 14 ± 2 |
| ced-1(e1735); nuc-1(e1392) | 1.0 ± 1.0 | 0–3 | 26 ± 3 |
| ced-1(n1995); nuc-1(e1392) | 1.2 ± 1.0 | 0–5 | 27 ± 5 |
| ced-7(n2094); nuc-1(e1392) | 21.1 ± 1.3 | 15–28 | 38 ± 3 |
| ced-7(n1892); nuc-1(e1392) | 22.3 ± 3.2 | 14–29 | 37 ± 5 |
| ced-7(n1996); nuc-1(e1392) | 19.0 ± 1.7 | 16–26 | 38 ± 4 |
Embryos were scored at the 1.5-fold stage. The data shown are means ± s.e.m.