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. 2010 Sep 28;21(4):588–599. doi: 10.1038/cr.2010.137

Figure 6.

Figure 6

Cell membrane depolarization is a prerequisite for insulinotropic effect of Sioc145. (A) Cell membrane potential was monitored as bisoxonol fluorescence in INS-1E cells stimulated with Sioc145 (1 μM), PMA (0.05 μM) or PMA plus Gö6976 (1 μM), in the presence of 2.8 mM glucose. Arrow indicates the time point when indicated compounds were added. (B) INS-1E cells were treated with indicated combinations of Sioc145 (1 μM), PMA (0.05 μM), DZX (250 μM) and KCl (30 mM), in the presence of 2.8 mM or 16.7 mM glucose for 1 h. Data are means ± SEM from three independent experiments. N.S., not significant; *P < 0.05; **P < 0.01.