Figure 5.

Apoptosis induced by PMA and ionomycin for SATB1-null peripheral CD4 SP cells. CD4 SP T cells were isolated from lymph nodes of control (C, either +/+ or +/−) and SATB1-null (KO) mice as described in Materials and Methods. The +/+ and +/− mice gave identical results. (A) The FACS profile of the purified CD4 SP cells from wild-type lymph nodes is shown in the inset. In each well of a 96-well culture plate, 3 × 10⁴ cells were seeded and cultured in medium with or without PMA plus ionomycin. Cell number was determined at 24-, 48-, and 72-hr time points and the percentages of surviving cells are shown. The cell number did not vary >4% among the three samples. (▴) KO (med.); (█) KO (PMA+I); (⋄) C (med.); (□) C (PMA+I). (B) The IL-2 concentrations in the media were measured by ELISA assay at each time point after stimulation with PMA plus ionomycin. The IL-2 amounts per viable cell (pg/cell) are shown (the average values are shown by an open bar for control and a solid bar for KO CD4 SP T cells). The minimum and maximum pg/cell values obtained are indicated for each bar.