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ctf7 cells are delayed prior to anaphase onset. (A). The Western blot shows HA–Pds1p levels in wild-type and ctf7 cells that were synchronized in G1 and then released into medium and grown at 37°C for 3 hr. Tubulin levels verified that equal protein loads were present in each lane. (B) Micrographs show the localization of DNA (DAPI) and HA–Pds1p (HA–Pds1p) in ctf7 mutant and wild-type cells treated as in A.
