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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: J Immunol. 2009 Oct 14;183(9):5778–5787. doi: 10.4049/jimmunol.0901120

Figure 5. Sp1 interacts at the PRDM1 proximal promoter.

Figure 5

Electrophoretic mobility shift assay using an oligonucleotide spanning the Sp1 consensus sequence identified at position −52 to −43 in the PRDM1 promoter. Lanes 1 and 2 contain 0 and 2 uL of nuclear extract respectively. The binding reactions in lanes 3 and 4 were incubated with the specific antibody indicated at the top of each lane. Unlabeled competitor oligonucleotides as indicated at the top of lanes 5–10 were added to the binding reaction at 150 or 300-fold molar excess. The sequence of the Sp1 oligonucleotide and the mutant probe are shown at the bottom of the figure. The Sp1 containing complex is indicated by the labeled arrowhead. The smaller arrowhead represents a related specific GC-box binding protein antigenically unrelated to Sp1.