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Region I sequences are sensitive to promoter DNA conformation. End-labeled σN (lanes 1–4) or holoenzyme prepared with end-labeled σN (lanes 5–7) were incubated for 10 min without DNA (lanes 2,5), with homoduplex DNA (lanes 3,6) or with heteroduplex DNA (lanes 4,7) and then cleaved with endoproteinase Glu-C (Casaz and Buck 1997). Numbers on the left indicate the amino acid at which cleavage has occurred.
