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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cell Calcium. 2006 Nov 27;41(6):559–571. doi: 10.1016/j.ceca.2006.10.006

Figure 1.

Figure 1

Temporal properties of local Ca2+ signals in neurons during phasic or repetitive firing. Series of successive x–y confocal fluorescence images were recorded at high speed (9.25 ms/frame) in cultured rat SCG neurons, loaded with fluo-4 AM and field stimulated to elicit Ca2+ transients. (A–B) Time courses of ΔF/F0 signals at non-nuclear and nuclear areas of interest (AOIs; indicated as white-colored zones) in two representative neurons showing a single or multiple Ca2+ transients in response to a single, 2 ms field stimulus (arrows). DAPI, a DNA dye was used to identify the nucleus more precisely. Scale bar 5 µm.