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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cell Calcium. 2006 Nov 27;41(6):559–571. doi: 10.1016/j.ceca.2006.10.006

Figure 7.

Figure 7

5 s trains of 1 Hz electrical field stimulation in the presence of BaCl2 successfully induce NFATc1-CFP translocation from cytosol to nucleus. (A–B) Representative images of neurons expressing NFATc1-CFP before (left), and 40 min after the start of electrical stimulation (right) using repetitive application (once every 50 s) of a 5 s 1Hz train in control conditions (top) or after the application of 2 mM Ba2+ (bottom). (C–D) 20–60 min application of staurosporin (5 µM) following the test condition in Figure 7A and B resulted in further increase in nuclear NFATc1. Scale bar 10 µm. Images from one respresentative neuron are shown in the top row of A and C, and from another neuron in the bottom row in A and C. n = 26 for the top row of B and D, and 34 for the bottom row of B and D. Bar plots, nuclear/cytoplasmic ratios (Fnuc/Fcyt) for each condition.