Figure 4. Antigen-specific T cells show limited effector function but have high effector potential in mycobacterial granulomas.
(A and B) Representative histograms gated on CD4+EGFP+ cells from p25-EGFP-bearing, BCG- or rBCG30-infected animals that had been injected with PBS or Ag85b peptide 2 hours prior to analysis or restimulated with P+I in vitro. (C and D) Quantification of the percentage and MFI of IFN-γ+ p25 T cells. Graphs show mean+/− SD from at least three mice per group and are representative of three similar experiments. P values from a two-tailed T test are shown. (D) Analysis IFNγ and TNFα expression by p25-EGFP. See also Figure S2.