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. 2011 Sep;80(3):407–415. doi: 10.1124/mol.110.070193

Fig. 5.

Fig. 5.

Mutation of S475A prevents CAMKIIα-dependent increases in Nox5 activity. A, COS-7 cells were cotransfected with either WT HA-Nox5 or S475A, S659A, or S502A mutants together with WT CAMKIIα or control DNA (lacZ), and basal superoxide was measured with L-012. Cell lysates were immunoblotted for relative expression of HA-Nox5 (bottom). Results are presented as mean ± S.E.M. (n = 4–5); *, p < 0.05 versus lacZ control. B, COS-7 cells were transfected with WT or S475A or S659A or S502A Nox5 and superoxide production was measured after stimulation with ionomycin (1 μM). Results are presented as mean ± S.E.M. (n = 5–6); *, p < 0.05 versus WT.