Fig. 1.

The UV-visible difference spectra of the carbonmonoxyl forms of the purified CYP2B6.4 to CYP2B6.9 polymorphic variants. The difference spectra were recorded according the method of Omura and Sato (1964). CYP2B6 WT and variants were reduced with a few grains of dithionite in 0.1 M potassium phosphate buffer, pH 7.4, containing 15% glycerol. The concentrations of CYP2B6 WT and CYP2B6.4 to CYP2B6.9 were 0.41, 0.28, 0.62, 0.60, 0.27, 0.32, and 0.53 μM, respectively.