TABLE 1.

The forward mutagenic primers used for preparation of the polymorphic mutants of CYP2B6

The base pairs that contain the desired mutations are underlined. The single mutants were prepared using the plasmid of CYP2B6*1 as templates, whereas the double and triple mutants were prepared using the plasmids of CYP2B6*4 and CYP2B6*6 as templates, respectively, as described under Materials and Methods.

CYP2B6	Amino Acid	Mutagenic Primers
CYP2B6*4	K262R	GACCCCAGCGCCCCCCGCGACCTCATCGACACC
CYP2B6*5	R487C	CAACATACCAGATCTGCTTCCTGCCCCGC
CYP2B6*6	Q172H/K262R	CYP2B6*4 + GACCCCACCTTCCTCTTCCATTCCATTACCGC
CYP2B6*7	Q172H/K262R/R487C	CYP2B6*6 + CAACATACCAGATCTGCTTCCTGCCCCGC
CYP2B6*8	K139E	AGGGACTTCGGGATGGGAGAACGGAGTGTGGAG
CYP2B6*9	Q172H	GACCCCACCTTCCTCTTCCATTCCATTACCGC