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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Anal Bioanal Chem. 2011 May 15;401(1):237–244. doi: 10.1007/s00216-011-5087-z

Fig. 1.

Fig. 1

A) Separation of disaccharides, GlcA-AManR and IdoA-AManR and estimation of epimer percentage in K5NS and CDSNS-heparin polysaccharides. Extracted ion chromatograms for m/z 339.1 of nitrous acid cleaved deaminative products of CDSNS-heparin (top) and K5NS (bottom). Both K5NS and CDSNS substrates were subjected to low pH nitrous acid mediated deaminative cleavage followed by sodium borohydride reduction and the resulting disaccharides were desalted before analysis by LC-MS. B) Mass spectrum of the disaccharides. Isotopic pattern of molecular ions corresponding to the disaccharides, HGlcA-AManR or HIdoA-AManR, carrying natural isotopic abundance is shown. Both the disaccharides have the same monoisotopic mass at m/z 339.1.