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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Anal Bioanal Chem. 2011 May 15;401(1):237–244. doi: 10.1007/s00216-011-5087-z

Fig. 2. DEEP-LC-MS analysis to characterize the action of HS C5-epimerase.

Fig. 2

Total ion chromatogram of epimerase treated K5NS in D2O is shown. After reaction with C5-epimerase the product was subjected to low pH nitrous acid treatment followed by sodium borohydride reduction. The resulting anhydromannitol disaccharide mixture was desalted using a mini Sephadex-G10 column (1cm × 10 cm) before MS analysis. The first and second peaks correspond to GlcA-AManR and IdoA-AManR, respectively (A, top panel); Mass spectrum of the first peak corresponding to the mixture of HGlcA-AManR and DGlcA-AManR (B, bottom left) and the second peak corresponding to DIdoA-AManR (C, bottom right). Isotopic pattern of a given molecular ion facilitated the estimation of reacted/unreacted disaccharide species.