Table 2.

Effect of the extracted sponges on clotting time. For RT, the extracts (50 μg/mL) were preincubated with plasma and then CaCl₂ was added to trigger coagulation. For PT, extracts (100 μg/mL) were incubated with plasma and then thromboplastin was added to induce coagulation. For aPTT, the extracts (100 μL/mL) were incubated with plasma plus cephalin then CaCl₂ was added to trigger coagulation. For FC, the extracts (100 μg/mL) were incubated with fibrinogen and then thrombin (1.0 U/mL) was added to induce coagulation. Results are expressed as means ± SEM of two individual experiments (n = 4). Asterisk used when p < 0.05 compared to NaCl or DMSO (1% v/v). See Table 4 for species names.

Samples	Clotting times (s)
	RT	PT	aPTT	FC
NaCl	124.3 ± 7.2	21.7 ± 0.3	32.1 ± 1.2	25.2 ± 0.1
DMSO	160.6 ± 11.5	22.7 ± 0.2	34.8 ± 1.3	34.4 ± 0.4
Pc	203.5 ± 4.7 *	27.4 ± 1.0 *	48.5 ± 1.4 *	86.6 ± 5.8 *
Ma	227.0 ± 8.0 *	27.1 ± 0.7 *	54.8 ± 2.0 *	99.8 ± 9.6 *
Da	210.6 ± 17.1	27.9 ± 1.2 *	52.3 ± 1.7 *	88.9 ± 6.9 *
De	197.2 ± 10.6	26.3 ± 0.4 *	54.1 ± 1.2 *	101.6 ± 6.0 *
Ti	246.0 ± 9.3 *	27.3 ± 0.8 *	64.2 ± 1.7 *	176.2 ± 4.7 *
Av	239.3 ± 14.8 *	27.1 ± 0.6 *	58.0 ± 1.6 *	94.4 ± 5.4 *
Hh	185.6 ± 10.1	23.9 ± 0.6	40.6 ± 1.1 *	76.6 ± 3.1 *
Af	234.6 ± 13.6 *	25.7 ± 0.5 *	45.2 ± 2.2 *	75.9 ± 5.4 *
Ch	296.3 ± 29.0 *	27.5 ± 0.4 *	53.2 ± 2.8 *	92.6 ± 8.1 *
Pj	278.6 ± 6.5 *	26.3 ± 0.5 *	52.2 ± 1.0 *	107.1 ± 5.0 *