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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Pediatr Blood Cancer. 2011 May 25;57(6):930–938. doi: 10.1002/pbc.23174

Figure 1. GLI1 and PTCH expression in human RMS cell lines.

Figure 1

The levels of GLI1 (A) and PTCH (B) mRNA were measured by RNase protection assay (RPA) using GAPDH as an internal control. The relative mRNA levels were extrapolated from a standard curve generated during the run. RPA expression of GLI1 was validated by qRT-PCR (C).