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PglB activity assays performed on CEFs of inter-transmembrane domain loop mutants. Each assay set included a negative control (‘Blank CEF’) and a positive PglB control (WT). Each plot indicates the mutants assayed in the legend. Shown here are those with mutations in the conserved loop motifs and the D475xxK motif. Assay results for negative-control mutants (K124A and K351A) and additional DxxK motifs are shown in Supporting Information (Figure S4).
