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. Author manuscript; available in PMC: 2012 Sep 6.
Published in final edited form as: Biochemistry. 2011 Aug 16;50(35):7557–7567. doi: 10.1021/bi201018d

Figure 7.

Figure 7

Average initial rates for low-activity PglB mutants assayed at three concentrations of polyprenyldiphosphate disaccharide substrate (0.01, 0.1, and 1.0 μM). Percent of WT rate for each mutant is determined at each concentration, with the average rate of WT set to 100%. Inset: magnification of R145A, D152E, and E316A values for clarity. Rate values shown are corrected for small variations in concentration, as determined by quantitative western blot analysis. Raw rate data can be found in the Supporting Information (Table S7).