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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Anticancer Drugs. 2011 Oct;22(9):905–912. doi: 10.1097/CAD.0b013e3283484750

Figure 1. PTEN reconstitution and c-Met inhibition decrease Akt activation and glioma cell growth responses.

Figure 1

U87-tetPTEN and U251-tetPTEN glioma cells were acclimated overnight in low serum (0.1% FBS), and then treated with the c-Met inhibitor SU11274 (10uM) ± doxycycline (2ug/mL). A, C) Immunoblot analysis of phospho-AktSer473 and total Akt were performed 24 hours after treatment. Cells were assayed 24, 48 or 72 hours after treatment for cell viability via MTT assay (B,D).