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. 1998 Jan 15;12(2):198–207. doi: 10.1101/gad.12.2.198

Figure 7.

Figure 7

Figure 7

The characterization of three mutant alleles and transformation rescue of the tir1-1 mutant with a candidate cDNA identify the TIR1 gene. (A) Predicted amino acid sequence of the TIR1 protein. The position of the tir1-1 and tir1-2 mutations, both (G → D), are indicated with an asterisk above the affected residue. The GenBank accession nos. for the cDNA and genomic sequences are AF005048 and AF005047, respectively. (B) RNA blot analysis of TIR1 transcript in tir1 mutants. Twenty-five micrograms of total RNA isolated from 13-day-old seedlings was loaded in each lane. (C) Transformation rescue of the tir1-1 mutant. The p35S:TIR1 plasmid was introduced into tir1-1 plants by vacuum infiltration. Transgenic lines were tested for auxin resistance by plating on medium containing 0.085 μm 2,4-D.