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. 1998 Jan 15;12(2):175–185. doi: 10.1101/gad.12.2.175

Figure 1.

Figure 1

 HIV-1 Vpr localizes to the nuclear envelope but point mutants are defective in localization. Indirect immunofluorescence on HeLa cells transfected with HA–Vpr expression constructs using an antibody to the HA–epitope tag on each Vpr expression construct. Staining for wild-type Vpr (A) is brightest in a ring outlining the nucleus; however, the point mutants, F34I (B) and H71R (C), are found in the nucleoplasm but not on the nuclear envelope. Optical sections are near the middle of each nucleus. Scale bar, 4 μm.