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. 1998 Jan 15;12(2):175–185. doi: 10.1101/gad.12.2.175

Figure 4.

Figure 4

 Vpr is expressed in yeast cells and colocalizes with the nuclear pore protein Nsp1p in yeast. Wild-type and mutant Vpr were expressed in wild-type yeast cells using a galactose-inducible promoter. Wild-type Vpr (top) colocalizes with the Nsp1p staining, whereas the F34I Vpr mutant (bottom) does not. Vpr was visualized using anti-Vpr polyclonal antibodies; Nsp1p was observed using an affinity-purified polyclonal antisera; DNA was visualized by DAPI staining and cells were viewed with Nomarski optics.