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. 1998 Jan 15;12(2):163–174. doi: 10.1101/gad.12.2.163

Figure 5.

Figure 5

 Southern blot analysis for CDKN2/p16 methylation. Flanking cut (F.C.) HUC was digested only with the enzyme for the F.C. EcoRI. All other samples were digested with both EcoRI and SacII. TSU–PR1 is a prostate cell line containing methylated CDKN2/p16 that was used as a positive control for methylation. The three TCC cell lines that expressed elevated p16 showed no altered methylation. In contrast, the three TCCs that failed to show p16 by Western blot analysis showed genetic alterations by Southern blot analysis. TCC 96-1 and TCC 97-1 both contained homozygous CDKN2/p16 deletions. TCC 94-10 had one methylated allele. Sequencing of cDNA showed a mutation in the second allele as discussed in text.