Figure 4.

Enhanced bacterial responses in cd200r^−/− mice. Tumor necrosis factor (TNF)–α (A) and interleukin (IL)–6 (B) liberated from wild-type (open bars) and cd200r^−/− (closed bars) alveolar macrophages 24 hours following incubation with varying multiplicities of infection (MOI) of Streptococcus pneumoniae. C, Total colony-forming units (CFU) recovered from the airway and lung tissue of wild-type (open bar) and cd200r^−/− mice (closed bar) at 12, 72, and 168 hours after 10⁴ CFU S. pneumoniae infection. D, Airway levels of TNF-α in wild-type (triangles) and cd200r^−/− mice (squares) 12 hours and 72 hours after S. pneumoniae infection. Cytokine data were analyzed using an unpaired 2-tailed Student t-test, assuming unequal variance. All other data were tested using a 2-tailed Mann–Whitney t test with 95% confidence intervals. Bonferroni correction was used for multiple comparisons. The data are representative of 4 independent experiments (n = 4 mice per group). * P < .05, ** P < .01, *** P < .001 versus corresponding group.