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. 1998 Jan 15;12(2):233–245. doi: 10.1101/gad.12.2.233

Figure 4.

Figure 4

Figure 4

 Interaction of GST–p300 with deletion variants of ATF-2. (A) Schematic representation of the ATF-2 fusion proteins synthesized in insect cells. (B) Extracts of infected insect cells containing wild-type or mutant ATF-2 proteins were incubated with immobilized GST–p300 fusion protein for 2 hr at 4°C. The beads were washed extensively and bound protein was analyzed by Western blotting analysis with antibodies specific for the amino- or carboxy-terminal-specific region of ATF-2 (lanes 3,6,9,12,15). Beads with bound GST protein (lanes 2,5,8,11,14) were used as a control. Lanes 1,4,7,10, and 13 (Load) were loaded with 0.5% (in terms of cpm) of the input recombinant ATF-2 or the variant proteins used in the binding experiments. The positions of ATF-2–FL, ATF-2–ΔN, ATF-2–Δ21, ATF-2–Δ9 and ATF-2-Δ(amino acids 112–350) are indicated by arrows.

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