TABLE 3.
SDD (mg/l) | GSH (nmol/mg) | GSSG (nmol/mg) | GSH/GSSG | ΔE (mV)a | ||||
Average | SEM | Average | SEM | Average | SEM | Average | SEM | |
Oral mucosa | ||||||||
0 | 43.23 | 2.47 | 4.40 | 0.22 | 9.92 | 0.73 | −206.97 | 1.62 |
0.3 | 45.12 | 5.07 | 5.12 | 0.52 | 8.82 | 0.60 | −205.68 | 2.35 |
4 | 44.75 | 1.84 | 4.53 | 0.25 | 9.97 | 0.56 | −207.60 | 1.12 |
14 | 47.02 | 5.41 | 5.37 | 0.62 | 8.77 | 0.38 | −206.24 | 1.87 |
60 | 43.17 | 5.13 | 4.90 | 0.56 | 8.79 | 0.15 | −205.06 | 2.06 |
170 | 46.34 | 5.63 | 5.67 | 0.80 | 8.26b | 0.19 | −205.29 | 1.35 |
520 | 46.38 | 4.57 | 5.88b | 0.46 | 7.88c | 0.32 | −204.78 | 1.59 |
Duodenum | ||||||||
0 | 16.77 | 1.26 | 0.24 | 0.03 | 71.33 | 5.15 | −220.58 | 1.36 |
0.3 | 17.57 | 1.25 | 0.30 | 0.03 | 59.99 | 6.85 | −218.77 | 2.03 |
4 | 16.99 | 0.73 | 0.30 | 0.01 | 57.33 | 0.49 | −218.11 | 0.56 |
14 | 16.75 | 0.94 | 0.31 | 0.01 | 53.41 | 2.69 | −216.86 | 1.44 |
60 | 17.61 | 1.08 | 0.36b | 0.01 | 48.47b | 2.00 | −216.24 | 1.36 |
170 | 18.92 | 1.49 | 0.43c | 0.01 | 44.28c | 2.72 | −215.89 b | 1.77 |
520 | 19.98 | 1.32 | 0.48c | 0.02 | 41.23c | 1.86 | −215.75b | 1.37 |
Jejunum | ||||||||
0 | 15.30 | 0.74 | 0.34 | 0.02 | 45.29 | 2.11 | −213.49 | 1.08 |
0.3 | 14.14 | 0.95 | 0.37 | 0.01 | 38.43 | 1.68 | −210.20 | 1.39 |
4 | 13.63 | 0.48 | 0.33 | 0.02 | 41.12 | 1.66 | −210.70 | 0.58 |
14 | 12.09 | 0.36 | 0.36 | 0.02 | 34.51d | 2.30 | −206.68 | 1.05 |
60 | 14.25 | 0.99 | 0.32 | 0.01 | 45.51 | 5.25 | −212.28 | 2.29 |
170 | 11.47c | 0.23 | 0.29 | 0.01 | 40.37 | 1.86 | −208.15c | 0.71 |
520 | 12.79b | 0.45 | 0.33 | 0.01 | 38.72 | 1.54 | −209.05b | 0.98 |
Plasma | GSH (μM) | GSSG (μM) | ||||||
0 | 25.45 | 0.74 | 3.59 | 0.35 | 7.34 | 0.65 | −137.40 | 1.26 |
0.3 | 24.86 | 0.55 | 4.18 | 0.27 | 6.05 | 0.46 | −134.59 | 1.17 |
4 | 27.69 | 1.53 | 4.20 | 0.33 | 6.84 | 0.92 | −137.35 | 2.17 |
14 | 26.91 | 1.45 | 4.04 | 0.32 | 6.92 | 0.83 | −137.10 | 2.44 |
60 | 32.45c | 1.37 | 4.18 | 0.23 | 7.93 | 0.81 | −141.62 | 1.80 |
170 | 30.41b | 1.09 | 4.85b | 0.23 | 6.35 | 0.45 | −137.90 | 1.46 |
520 | 31.46b | 1.82 | 5.07c | 0.23 | 6.28 | 0.53 | −138.12 | 1.84 |
See “MATERIALS AND METHODS” for calculation.
Statistically significant from control by Shirley’s tests (p < 0.05).
Statistically significant from control by Shirley’s tests (p < 0.01).
Statistically significant from control by Dunn’s tests (p < 0.01).