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Effect of leptin on p44/42 MAPK activation. p44/42 MAPK (p44/42) and phospho-p44/42 MAPK (P-p44/42) were detected in extracts of PMNLs from HS (A and B) and from patients on HD treatment (C and D) stimulated by Escherichia coli (A and C) or by PMA (B and D) incubated in the presence of 70 ng/mL leptin or buffer alone. Representative western blots and the densitometric evaluation of the blots are shown. Mean values ± SEM. n = 4–7; *P < 0.05 versus the absence of stimulus.
