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. 2011 Sep 1;22(17):2994–3009. doi: 10.1091/mbc.E11-04-0286

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© 2011 Gao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Elevation of M6P in the absence of ER stress releases free glycans. (A–D) PERK^−/− MEFs were incubated with (A, B) variable concentrations of mannose for 60 min; (C) 10 mM mannose for variable times; or (D) 10 mM mannose for 60 min. (A and C) Hexose-Ps (top) and free glycans (bottom) as in Figures 3B, 5A, and 6B. Black arrowheads indicate glycans increased by mannose treatment. (B) Quantitative measurements (averages of duplicates) of M6P (white) and total free glycans migrating in the G₄–G₇ standard range (black) from (A). (D) Lumenal and cytosolic free glycans from untreated or mannose-treated cells were separated, as in Figure 5D. Standard lanes are marked with asterisks. (E) As for (C), with PERK^+/+ MEFs.