Skip to main content
. 1998 Jan 15;12(2):149–162. doi: 10.1101/gad.12.2.149

Figure 3.

Figure 3

 Expression of VEGF mRNA in ES cells and cystic embryoid bodies. (A) ES cells. Hif1a+/+ and Hif1a−/− cells were incubated in complete medium at 20% O2 (N), complete medium at 1% O2 (H), or glucose-deficient medium at 20% O2 (−G) for 16 hr. Total RNA was isolated and analyzed by blot hybridization with 32P-labeled VEGF cDNA (top) and 18S rRNA oligonucleotide (bottom) probes. (B) Embryoid bodies. ES cells were cultured for 4 days in methylcellulose to induce differentiation and then exposed to 20% (N) or 0% (H) O2 for 16 hr prior to RNA isolation and blot hybridization as described above.