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. 2011 Sep 1;22(17):3022–3031. doi: 10.1091/mbc.E11-03-0196

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© 2011 Bartoccini et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — NLM in embryonic hippocampal cells. (A) Lipid composition compared with that of NFL and nuclei (N). The data are expressed as μg/mg protein and represent the average ± SD of three experiments performed in duplicate. The values of each lipid in nuclei and in the NLM are different with respect to those of NFL (significance, **p < 0.001). (B) SMase activity compared with that of NFL and nuclei (N). The data are expressed as pmol/mg protein/min and represent the average ± SD of three experiments performed in duplicate. The values for nuclei and NLM are different with respect to those of NFL (significance, **p < 0.001). (C) Immunoblot of STAT3 as purification marker of NML. Seven fractions (1–5 density gradient fractions) plus two floating fractions (6–7) were obtained, and immunoblotting for STAT3 was performed as described in Materials and Methods. The position of the 92-kDa protein was indicated in relation to the position of molecular size standards.