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. 2011 Sep 1;22(17):3022–3031. doi: 10.1091/mbc.E11-03-0196

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© 2011 Bartoccini et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Effect of physiological concentration of vitamin D₃ in normal and serum-deprivation condition. (A) PC synthesis and (B) SM synthesis in NFL after 2 h. The data indicating [³H]palmitic acid incorporation in PC and SM are expressed as cpm/mg protein and represent the average ± SD of three experiments performed in duplicate (significance, **p < 0.001 vs. control sample). (C) SM content in NLM after 10 h of culture. The data are expressed as μg/mg protein and represent the average ± SD of three experiments performed in duplicate (significance, **p < 0.001 vs. control sample). (D) SMase activity in NLM after 10 h of culture. The data are expressed as cpm/mg protein/min and represent the average ± SD of three experiments performed in duplicate (significance, **p < 0.001 vs. control sample). C, control; SD, serum deprivation.