FIGURE 5:

Vitamin D₃ and VDR in NLM in normal and serum-deprivation condition. (A) VDR immunoblotting analysis was performed in whole cells and in NLM after 10 h of culture with 10% FBS (C, control) without or with 100 nM vitamin D₃ or in serum-deprivation condition (SD) or in serum deprivation in the presence of physiological concentration of vitamin D₃ (SD + 100 nM vit. D3) or in serum deprivation in the presence of high concentration of vitamin D₃ (SD + 400 nM vit D3). The position of the 50-kDa protein was indicated in relation to the position of molecular size standards. Area density evaluated by densitometry scanning and analyzed with Scion Image are shown on the right. The data represent the average ± SD of three experiments performed in duplicate (significance, ** p < 0.001 vs. control sample). (B) Comparison of 100 nM [³H]vitamin D₃ incorporation in NLM purified from the cells cultured for 10 h with 10% FBS (C, control) or in serum-deprivation condition (SD), and of 400 nM [³H]vitamin D₃ incorporation in NLM purified from the cells cultured for 10 h in serum-deprivation condition. The data are expressed as cpm/mg protein and represent the average ± SD of three experiments performed in duplicate (significance, **p < 0.001 vs. control sample).