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. 2011 Sep 1;22(17):3055–3069. doi: 10.1091/mbc.E11-01-0069

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© 2011 Xiao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Changes in CUGBP1 and miR-222 expression after altering the levels of cellular polyamines. (A) Changes in the levels and cellular distribution of CUGBP1 and TIAR proteins after decreasing cellular polyamines by inhibiting ODC with DFMO (left) or after increasing polyamine levels by ectopic ODC overexpression (right) in stable ODC-IEC cells. Whole-cell, cytoplasmic, and nuclear lysates were prepared for Western blotting. Equal loading was monitored by β-actin in total lysates, β-tubulin in cytoplasmic fractions, and lamin B in nuclear fractions. (B) Changes of global miRNA profile (top) as measured by miRNA array and the levels of miR-222 (bottom) as examined by Q-PCR analysis after polyamine depletion by treatment with DFMO. Values are means ± SE of data from three separate experiments. *p < 0.05 compared with control cells and cells exposed to DFMO plus putrecine (Put). (C) Changes of global miRNA profile (left) and miR-222 levels (right) in stable ODC-IEC cells. ⁺p < 0.05 compared with cells transfected with a control vector.