FIGURE 9:

Effect of CUGBP1/miR-222–regulated CDK4 expression on cell proliferation. (A) Flow cytometric analysis of cell-cycle distribution after miR-222 overexpression with or without CUGBP1 silencing. IEC-6 cells were transfected with control scrambled oligomer, premiR-222 alone, or premiR-222 plus siCUGBP1; 48 h later, cells were subjected to fluorescence-activated cell sorting analysis. (B) The relative G1, S, and G2/M compartments were also calculated from data described in (A). Data are representative of three separate experiments. (C and D) Changes in the levels of CDK4 protein expression (C) and doubling time of cell growth (D) in ODC-IEC populations. (E and F) Effect of CDK4 silencing on cell growth in stable ODC-IEC cells. After cells were transfected with either control siRNA (C-siRNA) or siRNA targeting CDK4 mRNA (siCDK4) for 48 h, the levels of CDK4 protein (E) and cycle distribution (F) were examined. Three separate experiments were performed that showed similar results.