FIGURE 2:

Msn2 activity in 35 Msn2 partner–deleted strains generated on the S288c background analyzed by flow cytometry measurements of Hsp12-GFP reporter gene expression. The Hsp12-GFP expression level in the different strains was measured following yeast exposure to four different stress conditions and normalized to the wild-type strain (see Supplemental Figure S2 for examples and Supplemental Table S2 for raw data). The yeast cells were exposed to 40 min of stress, including oxidative stress (0.6 mM H₂O₂), osmotic stress (0.5 M NaCl), and heat stress (temperature shift from 30 to 37°C). Msn2 partner deletions that result in at least a 30% decrease or increase in Hsp12-GFP expression levels relative to the nondeleted strain led to the identification of the absent protein as a Msn2 activator or suppressor, highlighted in green and red, respectively. The glc7, cyr1, tor2, and cdc25 mutant strains contained a DaMP fusion (see Materials and Methods and Supplemental Table S5).