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. 2011 Sep 1;22(17):3218–3230. doi: 10.1091/mbc.E10-11-0874

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© 2011 Eyster et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — CD44, CD98, MHCI, and TfnR show increased localization with EEA1 in MARCH-expressing cells. HeLa cells transfected with MARCH1-FLAG (B), MARCH4-FLAG (C), or MARCH8-FLAG (A, D) were identified as in Figure 4 and are indicated with an asterisk. Primary antibodies to CD44 (A), CD98 (B), MHCI (C), or TfnR (D) were added to cells and allowed to internalize for 30 min. Mouse antibodies to CD44, CD98, MHCI, and TfnR were detected with anti–mouse Alexa 488 (green) and rabbit anti-EEA1 with anti–rabbit Alexa 594 (red). Bar, 10 μm. (E) The percentage of each cargo colocalizing with EEA1 in the absence (black bars) or presence (clear bars) of the indicated MARCH protein was quantified using Metamorph software. **Statistical significance at p < 0.001 between indicated cargo in control (black bars) and MARCH-expressing cells (clear bars). Bars, 10 μm.