Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Sep 1;22(17):3253–3262. doi: 10.1091/mbc.E11-04-0314

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Frieser et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 5: — Expression of Cla4-resistant Cdc24* interferes with induction of filamentous growth. (A) The relative concentration of Cdc24-3xHA and Cdc24*-3xHA in which all serines and threonines within the critical region for destabilization were mutated into alanines was determined before (0 h) and after 8 h of bE1/bW2 overexpression. Shown are the mean values of three independent experiments. SDs are reflected by the bars. (B) Phenotype of U. maydis cells expressing either wild-type Cdc24 or Cla4-resistant Cdc24* at the endogenous locus. Scale bars, 10 μm. (C) Quantification of length and diameter of the cells shown in B (n = 50). (D) Quantification of the percentage of cells without filaments and cells with filaments of indicated length (shorter than 10 μm, between 10 and 20 μm, between 20 and 50 μm, and longer than 50 μm) 4 h after the induction of bE1/bW2 overexpression in strains expressing wild-type Cdc24 or mutated Cdc24* at the endogenous locus (n = 100). (E) Representative images of cells from the cultures quantified in D. Pictures were taken 4 h after bE1/bW2 induction. Bars, 10 μm.