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. 2011 Sep 1;22(17):3263–3275. doi: 10.1091/mbc.E11-01-0020

FIGURE 4:

FIGURE 4:

The up-regulation of the expression of several genes involved in growth arrest following RhoC silencing depends on NAG-1. Real-time quantitative PCR analysis of the expression of the indicated mRNA was performed with total RNA extracted from PC-3 cells 48 h after transfection with 20 nM (or 40 nM) of specific controls for siRhoC (ctrC or ctrC[2X]), with 20 nM of siRhoC, or with 20 nM siRhoC + 20 nM of siNAG-1 (siRhoC + siNAG1). Results are the mean ± SD of three independent experiments. *p < 0.05, **, p<0.01, ***p < 0.001, ANOVA, followed by Tukey–Kramer analysis.