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. 2011 Sep 1;22(17):3263–3275. doi: 10.1091/mbc.E11-01-0020

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© 2011 Giang Ho et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Overexpression of RhoA, but not of RhoC, inhibits anchorage-independent growth. PC-3 clones overexpressing RhoC (A) or RhoA (B) and a control clone (PC-3/TR/T0) (B) were plated in soft agar as described in Materials and Methods. Cells were supplemented (+dox) or not with weekly renewed doxycycline (100 ng/ml). After 2 wk of culture, colonies were counted in the whole dishes. Results are reported on the unsupplemented condition and are expressed as mean ± SD. Representative results of three independent experiments are shown. ***p < 0.001, ANOVA, followed by Tukey–Kramer analysis.