Figure 3.

Targeting of TRPM1 to dendritic tips of ON-BCs requires mGluR6. A, Colocalization of TRPM1 and mGluR6 at dendritic tips of ON-BCs. Cross sections of mouse retinas were double stained with TRPM1 (green) and mGluR6 (red) antibodies, as described in Materials and Methods. Scale bar, 5 μm. B, TRPM1 is absent at the tips of the ON-BC dendrites in retinas lacking mGluR6. Immunohistochemical analysis of TRPM1 localization in the outer plexiform layer of wild-type (WT), nob3, and nob4 retinas. Sections were double stained with TRPM1 (green) and presynaptic marker CtBP2 (red) antibodies. Insets show the outer plexiform layer at high magnification. Scale bar, 5 μm.