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Regulation of cell-specific gene expression in car4 null cells by LiCl. Wild-type JH10 and car4 null cells were pulsed with cAMP for 4.5 hr. The car4 null culture was split and adjusted to 0, 2, 5, or 10 mm LiCl. Pulsing was continued for 30 min, and all cultures were treated with 300 μm cAMP. After 5 hr RNAs were isolated and hybridized on Northern blots to the prestalk probe ecmB and the prespore probe cotB.
