Figure 3.

Biochemical properties of constructs normalized to wet weight (white bars) and per construct (shaded bars). A) GAG. Increased seeding led to significant increases in GAG/construct, whereas all constructs seeded with 3.75×10⁶ cells or greater had a significant increase in GAG production over the lowest seeding group when normalized to wet weight. B) Total collagen. No significant differences were observed between any treatment group for collagen/construct, while significant decreases in collagen/ww were observed with increased seeding. Data are shown mean ± standard deviation and significance defined as p < 0.05. Groups not connected by the same letter are deemed significantly different and capital letters denote differences between biochemical content normalized to wet weight and lowercase letters denote differences when normalized per construct.