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SPR analysis of the binding of SHP-2 N-SH2 domain (10 µM) to immobilized peptide biotin-(miniPEG)2-RVIYFVPLNR-NH2 in the presence of varying concentrations of the pYFVP peptide (0, 1.0, 2.1, 4.2, and 8.4 µM). (A) Overlaid sensograms at indicated pYFVP concentrations. (B) Plot of RUeq as a function of pYFVP peptide concentration. The curve was fitted to the data according to the equation: RUeq = RUmax[pY]/(KDapp + [pY]).
