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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Anal Biochem. 2011 Jun 30;417(2):211–219. doi: 10.1016/j.ab.2011.06.030

Table 2.

Oligonucleotides used in the vertical PCR/ExoSAP-IT®/LDR analyzer.

Oligomers Sequences (5′ – 3′) Tm (°C)d
K-ras coding-exon 1 forward CTCCCAAGGAAAGTAAAGTTCCCATA 58
K-ras coding-exon 1 reverse GGTACTGGTGGAGTATTTGATAGTGT 57.5
K-ras c12 com-2a pb TGGCGTAGGCAAGAGTGCCT-IRDye800c 63.5
dOligo-K-ras c12.2Da GCTGATGGCAGGTGCTGAAACTTGTGGTAGTTGGAGCTGA 69.7
dOligo -K-ras c12.2Aa TGACGTGACGAAACTTGTGGTAGTTGGAGCTGC 66.8
dOligo -K-ras c12.2Va TGACGTGGCTGAGGTCGGTCGCAGATGCTGAAAACTTGTGGTAGTTGGAGCTGT 72.9
a

Oligonucleotides are derived from Hashimoto et al. [28]. Bold letters represent stuffer sequences which generate different lengths of discriminating oligomers.

b

Phosphorylated.

c

λex = 787 nm and λem = 812 nm.

d

Melting temperature is calculated by oligo analyzer (http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/) using the following conditions: oligo concentration, 1 μM; Na+ concentration, 50 mM.