Table 2.
Oligomers | Sequences (5′ – 3′) | Tm (°C)d |
---|---|---|
K-ras coding-exon 1 forward | CTCCCAAGGAAAGTAAAGTTCCCATA | 58 |
K-ras coding-exon 1 reverse | GGTACTGGTGGAGTATTTGATAGTGT | 57.5 |
K-ras c12 com-2a | pb TGGCGTAGGCAAGAGTGCCT-IRDye800c | 63.5 |
dOligo-K-ras c12.2Da | GCTGATGGCAGGTGCTGAAACTTGTGGTAGTTGGAGCTGA | 69.7 |
dOligo -K-ras c12.2Aa | TGACGTGACGAAACTTGTGGTAGTTGGAGCTGC | 66.8 |
dOligo -K-ras c12.2Va | TGACGTGGCTGAGGTCGGTCGCAGATGCTGAAAACTTGTGGTAGTTGGAGCTGT | 72.9 |
Oligonucleotides are derived from Hashimoto et al. [28]. Bold letters represent stuffer sequences which generate different lengths of discriminating oligomers.
Phosphorylated.
λex = 787 nm and λem = 812 nm.
Melting temperature is calculated by oligo analyzer (http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/) using the following conditions: oligo concentration, 1 μM; Na+ concentration, 50 mM.