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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Stem Cells. 2010 Sep;28(9):1476–1486. doi: 10.1002/stem.470

Figure 1. Zap70 expression in mESCs.

Figure 1

(A) Zap70 mRNA levels from EL4 and mES were compared by qRT-PCR analyses. *, p < 0.05. (B) Relative levels of Zap70 mRNA from EL4, mES, and MEF cells were compared by RT-PCR analysis of total RNA. GAPDH was used as control of equal mRNA loading. (C) Levels of Zap70 mRNA at the indicated time after retinoic acid–induced differentiation, determined by RT-PCR analysis. GAPDH was used as an equal loading control for RT-PCR. (D) Top panel: Zap70 protein levels from whole-cell extracts of EL4, mES, and MEF cells compared by immunoblotting analysis. Middle panel: Oct4 immunoblotting indicates pluripotency of mESC. Bottom panel: β-actin was used as a loading control.