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. Author manuscript; available in PMC: 2012 Aug 6.
Published in final edited form as: Mol Cell Endocrinol. 2011 Jun 1;342(1-2):64–72. doi: 10.1016/j.mce.2011.05.036

Fig. 7.

Fig. 7

Differential induction of phospho-Smad3 isoform protein by activin in LbT2 and αT3-1 gonadotrope cells. Immunoblots of vehicle, activin A or activin B-treated αT3-1 or LβT2 cell lysates, using either anti-phospho-Smad3 antibody (top) or anti-Smad3 antibody (bottom). Activin treatment induced full-length phospho-Smad3 (52 kDa) and the phospho-Smad3 isoform (about 30 kDa) in LbT2 cells but not αT3-1 cells. There was also no unphosphorylated form of the Smad3 isoform protein present in αT3-1 cells.